Original Research Paper

Storage artefacts in peripheral blood film of anticoagulated blood

  Abstract :

Introduction:Examination of blood smears and hematologicparameters is often the first step in assessment ofhematologic function and diagnosis of related diseases. EDTA is the preferred anticoagulant for bloodcounts as it produces complete anticoagulation withminimal morphological alterations of cell. Aims and objectives: The current studywas undertaken to identify the anticoagulant induced artefactsand thus avoid misinterpretation of peripheral bloodsmears. Materials and methodsBlood samples were obtained from Hematologylaboratory at District Hospital, Samba. 100 blood samples werecollected directly into commercially preparedvaccutiners which contain correct concentration of K3EDTA as anticoagulant. Smears were madeimmediately as well as after 2 ,4, 6 hours ofstorage. Morphological artefacts were studied. Results and observations: Smears madeimmediately after addition of anticoagulant did notshow any artefactual changes. Smears from EDTA stored blood show significant morphologicalartefacts on storage. ConclusionEDTA has been recommended as the anticoagulant ofchoice for peripheral smear as it allows the bestpreservation of cellular components and morphology ofblood cells up to 2 hours of storage. After 2hrs itshows storage artifacts .

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