Original Research Paper

Production of cellulase enzyme by Aspergillus niger under solid state fermentation

K. Olikkavi, L. Vennila

  Abstract :

Filamentous fungi are important due to their high enzyme production potential. Many enzymes produced by fungi have been related to biotechnological applications in several industrial sectors. The purpose of this study was to isolate filamentous fungi from soil using rice bran,paddy husk and chaffer grains as a feed substrate and to screen for cellulase production potential. The cellulase enzyme extract was partially purified and its protein fraction was stained and molecular weight was determination by using SDS-PAGE. In this study Aspergillus niger were subjected for Solid State Fermentation (SSF) for the production of cellulose. These fungi showed maximum cellulase activities at 72 hours of incubation. The physical and nutritional parameters of fermentation like pH, temperature, substrate, carbon and nitrogen sources were optimized. The optimal conditions for maximum biosynthesis of cellulase by A. niger were found to be at pH 6 and temperature 30C. Among the three substrates, rice bran showed maximum cellulase production followed by paddy husk and chaffer grains. The present investigation paved the way for the production of fungal cellulase enzymes suitable for various industrial applications.

  Cite This Article:

PRODUCTION OF CELLULASE ENZYME BY ASPERGILLUS NIGER UNDER SOLID STATE FERMENTATION, K. Olikkavi, L. Vennila, INTERNATIONAL JOURNAL OF RESEARCH IN PATHOLOGY AND MICROBIOLOGY : Volume-5 | Issue-1 | January-2021

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