Original Research Paper
ANALYSIS OF THE LIPOLYTIC POTENTIAL OF THREE BACTERIAL STRAINS ISOLATED FROM BENTHIC SOIL OF AN AQUACULTURE FARM IN EAST KOLKATA WETLANDS, INDIA.
Prokaryotic species in the soil microenvironment perform a variety of activities in the circulation of nutrients by breaking down complex detritus molecules into simpler ones by the production of extracellular enzymes. In this study, three bacterial strains were isolated from benthic soil of an aquaculture farm in East Kolkata Wetlands, India, with considerable lipolytic potential. Upon molecular characterization using 16S rDNA analysis the strains were identified as Enterobacter cloacae Strain P4cR1, Klebsiella pneumoniae Strain P5aR1, and Serratia marcescens Strain P5bR1. Enzymatic analyses revealed that S. marcescens Strain P5bR1 shows the best enzymatic activity with 16.820.87 IU ml-1min-1 followed by K. pneumoniae Strain P5aR1 with 12.531.84 IU ml-1min-1 and E. cloacae Strain P4cR1 showing the lowest activity at 9.481.05 IU ml-1min-1. The enzymes were stable at a pH range of 7 to 8 and a temperature range within 30 to 40C. This is probably the first report of bacterial strains with lipolytic potential isolated from the benthic soil of an aquaculture farm. These bacteria may find their uses not only in bioremediation purposes but in industrial applications also.
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