Original Research Paper
Phenotypic detection, biofilm production and molecular characterization of E.coli isolates recovered from Blood stream infections in a tertiary care hospital
Objective: To examine Carbapenem resistance, & biofilm production in E.coli isolates recovered from BSIs.Methods: A prospective, Cross-sectional study was conducted in a 650 bedded tertiary care centre in Jhajjar, Haryana India from July 2019 to April 2020. A total of 79 isolates were recovered from Blood stream infections (BSIs). Carbapenem resistance, biofilm production was checked phenotypically and the presence of -lactamase genes by PCR. Isolate relatedness was determined by REP PCR, and ERIC PCR.Results: From July 2019 to April 2020, in this study a total of 79 E.coli isolates recovered from BSIs, 63 isolates were ESBL producers positive for TEM, SHV and CTX-M genes,48 were carbapenemase producers and 36 were metallo beta lactamase producers positive for DDST, CDST and MBL (IP/IPI) E-test. Out of these 36 MBL producers15 had blaNDM-1 gene, 11 had blaVIM-2 gene and 10 had blaIMP-1 gene respectively.Conclusions: The significant presence of both metallo beta lactamase genes and biofilm co-existence in the same isolates is worrisome.
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