Original Research Paper

Microbiology of diabetic foot determined by biopsy study of 100 patients.

  Abstract :

Objectives: The goal is to identify the microorganisms present in wounds on diabetic feet by analyzing biopsy samples.Materials and methods: Consecutive patients with diabetic foot and symptoms of infection were enrolled in the study. Biopsy samples were homogenized using a mortar and processed to identify aerobic organisms. When multiple isolates were found, only the two most common types of bacteria were identified. Bacterial identification was done using biochemical techniques, while the sensitivity to antibiotics was determined using the disk diffusion method.Results: A total of 100 biopsy samples were analyzed, with 52 of them taken from male patients (52%). Of the 102 isolates identified, 67% were Gram-negative bacilli, with Escherichia coli being the most common type, accounting for 21% of all isolates. Gram-positive cocci accounted for 28% of all isolates, while 6% were yeasts. Among the 68 Gram-negative bacilli, 35% were resistant to ciprofloxacin. The study found 55 Enterobacteriaceae isolates, 7% of which produced extended-spectrum beta-lactamases. Finally, the study identified 8 Staphylococcus aureus isolates, 38% of which were resistant to methicillin.Conclusions: When compared to reports from developed countries, our study identified a greater proportion of Gram-negative bacteria and antibiotic-resistant organisms.

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