Upregulation of Single nucleotide polymorphism of PD-1 gene (rs10204525) in chronic hepatitis B patients
Background: Programmed death-1 (PD-1) is major inhibitory molecule that reduces T cell activation. PD-1 and their polymorphisms have separately been shown to be associated with hepatitis B virus (HBV) infection. Therefore, we aimed to investigate the association of PD1 polymorphisms with potential of chronic hepatitis B infection in Iranian population to do a Case Control study.Methods: In this study HBV DNA, and Pd-1 mRNA, were extracted from 60 patients with chronic hepatitis B and 60 healthy blood donors as control. HBV Viral Load, polymorphism genotype of the two groups and level expression of Pd1 gene were evaluated using Real time PCR method. Results: Age mean of the control group was 40.73.7 and 35.8611.18 years old age for the case group. The GG allele between the two groups had the same frequency (73.3% in CHB group and 80% in healthy group). The GA allele in patients with hepatitis B (20%) was most frequent. There was a significant association between the GA allele and chronic hepatitis B infection. There was no significant relationship between the ages of the subjects with SNPrs10204525. Also, the expression of PD-1 mRNA was higher in people with hepatitis B than in healthy group.Conclusion: The results of this study showed that there is a significant relationship between the presence of rs10204525 polymorphisms in the PD-1 gene in patients with chronic hepatitis B infection and the association between the GA allele and chronic hepatitis B can be linked.
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